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What challenges are encountered in selecting appropriate internal standards for preclinical toxicology studies?

Master Preclinical Toxicology with HRMS

Unlock the highest level of confidence in your preclinical toxicology studies by harnessing the capabilities of High-Resolution Mass Spectrometry (HRMS). While traditional methods lack the sensitivity needed to distinguish subtle differences in mass-to-charge ratios (m/z) between ions, particularly on low-resolution instruments, HRMS provides a substantial advantage, resulting in robust results that surpass conventional methods.

Predict Toxicity Earlier in Development

HRMS enhances quantitative bioanalytical studies, including biomarker analysis & validation, enriching drug metabolism and pharmacokinetic (DMPK) assessments, and time profiles. It enables you to achieve the sensitivity and precise calibration necessary to accurately reflect both subtle and significant effects of your products on endogenous molecules at preclinical stages of development.

Overcome Matrix Effects

Labs often deal with problems like interference, cross-reactivity, and choosing internal standards for accurate calibration in qualitative & quantitative bioanalytical studies. HRMS effectively addresses the challenges posed by matrix effects and the fluctuating ionization and fragmentation efficiency of various compound classes in complex mixtures. Discover how Sannova's Z factor approach and good laboratory practices (GLP) synergise with HRMS methods to ensure that no significant effect evades detection.

Discover Expert Methods

With 17 years of experience in supporting emerging and mid-size Pharma and Biotech from preclinical through clinical phases, Sannova Analytical, a leading contract research organization (CRO), has developed 300+ validated methods across 21 species and matrices. Our whitepaper is a valuable guide for pharmaceutical and biotech developers seeking to extend the lower limit of quantification to measure endogenous molecules and select the right method for traditional and biological drug development. 

Download the Whitepaper